PT  - JOURNAL ARTICLE
AU  - Behnam Yousefi
AU  - Federico Melograna
AU  - Gianluca Galazzo
AU  - Niels van Best
AU  - Monique Mommers
AU  - John Penders
AU  - Benno Schwikowski
AU  - Kristel van Steen
TI  - Capturing the dynamics of microbiomes using individual-specific networks
AID  - 10.1101/2023.01.22.525058
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.01.22.525058
4099  - http://biorxiv.org/content/early/2023/01/22/2023.01.22.525058.short
4100  - http://biorxiv.org/content/early/2023/01/22/2023.01.22.525058.full
AB  - Background Longitudinal analysis of multivariate individual-specific microbiome profiles over time or across conditions remains a daunting task. The vast majority of statistical tools and methods available to study the microbiota are based upon cross-sectional data. Over the past few years, several attempts have been made to model the dynamics of bacterial species over time or across conditions. However, the field needs novel views on how to incorporate individual-specific microbial associations in temporal analyses when the focus lies on microbial interactions.Results Here, we propose a novel data analysis framework, called MNDA, to uncover taxon neighbourhood dynamics that combines representation learning and individual-specific microbiome co-occurrence networks. We show that tracking local neighbourhood dynamics in microbiome interaction or co-occurrence networks can yield complementary information to standard approaches that only use microbial abundances or pairwise microbial interactions. We use cohort data on infants for whom microbiome data was available at 6 and 9 months after birth, as well as information on mode of delivery and diet changes over time. In particular, MNDA-based prediction models outperform traditional prediction models based on individual-specific abundances, and enable the detection of microbes whose neighbourhood dynamics are informative of clinical variables. We further show that similarity analyses of individuals based on microbial neighbourhood dynamics can be used to find subpopulations of individuals with potential relevance to clinical practice. The annotated source code for the MNDA framework can be downloaded from: https://github.com/H2020TranSYS/microbiome_dynamicsConclusions MNDA extracts information from matched microbiome profiles and opens new avenues to personalized prediction or stratified medicine with temporal microbiome data.Competing Interest StatementThe authors have declared no competing interest.