RT Journal Article
SR Electronic
T1 Chitinase-like proteins promoting tumorigenesis through disruption of cell polarity via enlarged endosomal vesicles
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.08.17.504232
DO 10.1101/2022.08.17.504232
A1 Khalili, Dilan
A1 Kunc, Martin
A1 Herbrich, Sarah
A1 Mueller, Anna
A1 Theopold, Ulrich
YR 2023
UL http://biorxiv.org/content/early/2023/01/24/2022.08.17.504232.abstract
AB Chitinase-like proteins (CLPs) are associated with tissue-remodeling and inflammation but also with several disorders, including fibrosis, atherosclerosis, allergies, and cancer. However, CLP’s role in tumors is far from clear. Here, we utilize Drosophila melanogaster to investigate the function of CLPs (imaginal disc growth factors; Idgf’s) in RasV12 dysplastic salivary glands. We find one of the Idgf’s members, Idgf3, is transcriptionally induced in a JNK-dependent manner via a positive feedback loop mediated by reactive oxygen species (ROS). Moreover, Idgf3 accumulates in enlarged endosomal vesicles (EnVs) that promote tumor progression by disrupting cytoskeletal organization. The process is mediated via the downstream component, αSpectrin, which localizes to the EnVs. Our data provide new insight into CLP function in tumors and identifies specific targets for tumor control.Competing Interest StatementThe authors have declared no competing interest.