RT Journal Article
SR Electronic
T1 A simple image processing pipeline to sharpen topology maps in multi-wavelength interference microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.01.12.523706
DO 10.1101/2023.01.12.523706
A1 Peter W. Tinning
A1 Jana K. Schniete
A1 Ross Scrimgeour
A1 Lisa S. Kölln
A1 Liam M. Rooney
A1 Trevor J. Bushell
A1 Gail McConnell
YR 2023
UL http://biorxiv.org/content/early/2023/01/26/2023.01.12.523706.abstract
AB Multi-wavelength standing wave (SW) microscopy and interference reflection microscopy (IRM) are powerful techniques that use optical interference to study topographical structure. However, the use of more than two wavelengths to image the complex cell surface results in complicated topographical maps and it can be difficult to resolve the three-dimensional contours. We present a simple image processing method to reduce the thickness and spacing of antinodal fringes in multi-wavelength interference microscopy by up to a factor of two to produce clearer and more precise topographical maps of cellular structures. We first demonstrate this improvement using model non-biological specimens, and we subsequently demonstrate the benefit of our method for reducing the ambiguity of surface topography and revealing obscured features in live and fixed cell specimens.Competing Interest StatementThe authors have declared no competing interest.