PT  - JOURNAL ARTICLE
AU  - Huang, Sherri
AU  - Holmes, Michael J.
AU  - Radke, Joshua B.
AU  - Hong, Dong-Pyo
AU  - Liu, Ting-Kai
AU  - White, Michael W.
AU  - Sullivan, William J.
TI  - <em>Toxoplasma gondii</em> AP2IX-4 regulates gene expression during bradyzoite development
AID  - 10.1101/104208
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 104208
4099  - http://biorxiv.org/content/early/2017/01/30/104208.short
4100  - http://biorxiv.org/content/early/2017/01/30/104208.full
AB  - Toxoplasma gondii is a protozoan parasite of great importance to human and animal health. In the host, this obligate intracellular parasite persists as a tissue cyst form that is invisible to the immune response and unaffected by current therapies. The tissue cysts facilitate transmission through predation and give rise to chronic cycles of toxoplasmosis in immune compromised patients. Transcriptional changes accompany conversion of the rapidly replicating tachyzoites into the encysted bradyzoites, yet the mechanisms underlying these alterations in gene expression are not well-defined. Here we show that AP2IX-4 is a nuclear protein exclusively expressed in tachyzoites and bradyzoites undergoing division. Knockout of AP2IX-4 had no discernible effect on tachyzoite replication, but resulted in a reduced frequency of tissue cyst formation following alkaline stress induction – a defect that is reversible by complementation. AP2IX-4 has a complex role in regulating bradyzoite gene expression, as many bradyzoite mRNAs dramatically increased beyond normal stress-induction in AP2IX-4 knockout parasites exposed to alkaline media. The loss of AP2IX-4 also resulted in a modest virulence defect and reduced cyst burden in chronically infected mice, which was also reversed by complementation. These findings illustrate that the transcriptional mechanisms responsible for tissue cyst development operate across the intermediate life cycle from the dividing tachyzoite to the dormant bradyzoite.