PT  - JOURNAL ARTICLE
AU  - Sonia Singhal
AU  - Paul E. Turner
TI  - Effects of Historical Coinfection on Host Shift Abilities of Exploitative and Competitive Viruses
AID  - 10.1101/105114
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 105114
4099  - http://biorxiv.org/content/early/2017/02/01/105114.short
4100  - http://biorxiv.org/content/early/2017/02/01/105114.full
AB  - Rapid evolution contributes to frequent emergence of RNA viral pathogens on novel hosts. However, accurately predicting which viral genotypes will emerge has been elusive. Prior work with lytic RNA bacteriophage f6 (family Cystoviridae) suggested that evolution under low multiplicity of infection (MOI; proportion of viruses to susceptible cells) selected for greater host exploitation, while evolution under high MOI selected for better intracellular competition against co-infecting viruses. We predicted that phage genotypes that experienced 300 generations of low MOI ecological history would be relatively advantaged in growth on two novel hosts. We inferred viral growth through changes in host population density, specifically by analyzing five attributes of growth curves of infected bacteria. Despite equivalent growth of evolved viruses on the original host, low MOI evolved clones were generally advantaged relative to high MOI clones in exploiting novel hosts. We also observed genotype-specific differences in clone infectivity: High fitness genotypes on the original host also performed better on novel hosts. Our results indicated that traits allowing greater exploitation of the original host correlated positively with performance on novel hosts. Based on infectivity differences of viruses from high versus low MOI histories, we suggest that prior MOI selection can later affect emergence potential.