TY - JOUR T1 - Complex petal spot formation in the Beetle Daisy (<em>Gorteria diffusa</em>) relies on spot-specific accumulation of malonylated anthocyanin regulated by paralogous GdMYBSG6 transcription factors JF - bioRxiv DO - 10.1101/2023.02.20.529304 SP - 2023.02.20.529304 AU - Róisín Fattorini AU - Farahnoz Khojayori AU - Gregory Mellers AU - Edwige Moyroud AU - Eva Herrero Serrano AU - Roman T Kellenberger AU - Rachel Walker AU - Qi Wang AU - Lionel Hill AU - Beverley J Glover Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/02/21/2023.02.20.529304.abstract N2 - Gorteria diffusa has elaborate petal spots that attract male bee-fly pollinators through sexual deception but the genetic basis of G. diffusa petal spot development is currently unknown. Here we investigate the regulation of pigmentation during spot formation.We used UHPLC-MS/MS to determine the anthocyanin composition of spots and background pigmentation in G. diffusa. Combining gene expression analysis with protein interaction assays we characterised three R2R3-MYB genes regulating anthocyanin production in G. diffusa spots.We found that cyanidin 3-glucoside pigments G. diffusa ray floret petals. Unlike other petal regions, spots contain a high proportion of malonylated anthocyanin. We identified three paralogous subgroup 6 R2R3-MYB transcription factors that activate the production of petal spot pigmentation. The corresponding genes are upregulated in developing spots and induce ectopic anthocyanin production upon heterologous expression in tobacco. EMSAs and dual luciferase assays suggest that these transcription factors regulate genes encoding three anthocyanin synthesis enzymes: anthocyanidin synthase (GdANS), dihydroflavonol reductase (GdDFR) and malonyl transferase (GdMAT1), accounting for the spot-specific production of malonylated pigments.Here we provide the first molecular characterisation of G. diffusa spot development, showing that the elaboration of complex spots begins with accumulation of malonylated pigments at the base of ray floret petals, positively regulated by three subgroup 6 R2R3-MYB transcription factors.Competing Interest StatementThe authors have declared no competing interest. ER -