RT Journal Article
SR Electronic
T1 The use of DNA barcoding for the identification of giant hogweeds in the European North-East of Russia
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.02.21.529251
DO 10.1101/2023.02.21.529251
A1 D.M. Shadrin
A1 I.V. Dalke
A1 I.G. Zakhozhiy
A1 D.S. Shilnikov
A1 M.N. Kozhin
A1 I.F. Chadin
YR 2023
UL http://biorxiv.org/content/early/2023/02/22/2023.02.21.529251.abstract
AB Qualitative differences in the nrDNA ITS and ETS sequences in the total sample were determined to allow identification of the following groups of samples: H. mantegazzianum, H. cf. sosnowskyi, H. cf. mantegazzianum and H. cf. sosnowskyi × H. cf. mantegazzianum among other representatives of the Heracleum genus presented in the genetic databases. The study revealed the absence of correlation between a sample type in the group H. mantegazzianum, H. cf. sosnowskyi, H. cf. mantegazzianum and H. cf. sosnowskyi × H. cf. mantegazzianum and qualitative transformations in the ITS and ETS sequences. The qualitative analysis of the rbcL, matK, rps16 intron, intergenic spacers psbA-trnH, trnQ-rps16, rps16-trnK and rpl32-trnL cpDNA sequences for the samples identified as Heracleum mantegazzianum, H. cf. sosnowskyi, H. cf. mantegazzianum and H. cf. sosnowskyi × H. cf. mantegazzianum demonstrated no correlation between them as well.Competing Interest StatementThe authors have declared no competing interest.