TY - JOUR T1 - Cell-cycle status of male and female gametes during Arabidopsis reproduction JF - bioRxiv DO - 10.1101/2023.02.22.529524 SP - 2023.02.22.529524 AU - Yoav Voichek AU - Bohdana Hurieva AU - Caroline Michaud AU - Anna Schmücker AU - Zaida Vergara AU - Bénédicte Desvoyes AU - Crisanto Gutierrez AU - Viktoria Nizhynska AU - Benjamin Jaegle AU - Michael Borg AU - Frédéric Berger AU - Magnus Nordborg AU - Mathieu Ingouff Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/02/23/2023.02.22.529524.abstract N2 - Fertilization in Arabidopsis thaliana is a highly coordinated process that begins with a pollen tube delivering the two sperm cells into the embryo sac. Each sperm cell can then fertilize either the egg or the central cell to initiate embryo or endosperm development, respectively. The success of this double fertilization process requires a tight cell cycle synchrony between the male and female gametes to allow karyogamy (nuclei fusion). However, the cell cycle status of the male and female gametes during fertilization still remains elusive as DNA quantification and DNA replication assays have given conflicting results1–4. Here, to reconcile these results, we quantified the DNA replication state by DNA sequencing and performed microscopic analyses of fluorescent markers covering all the phases of the cell cycle. We show that male and female gametes in Arabidopsis are both arrested prior to DNA replication at maturity and initiate their DNA replication only during fertilization.Competing Interest StatementThe authors have declared no competing interest. ER -