RT Journal Article
SR Electronic
T1 A refined single cell landscape of haematopoiesis in the mouse foetal liver
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.03.01.530564
DO 10.1101/2023.03.01.530564
A1 Elena Ceccacci
A1 Emanuela Villa
A1 Fabio Santoro
A1 Saverio Minucci
A1 Christiana Ruhrberg
A1 Alessandro Fantin
YR 2023
UL http://biorxiv.org/content/early/2023/03/02/2023.03.01.530564.abstract
AB During prenatal life, the foetal liver is colonised by several waves of haematopoietic stem and progenitor cells (HSPCs) to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression pattern as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a novel scRNA-seq dataset from whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by HSPCs and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver.Competing Interest StatementThe authors have declared no competing interest.