PT - JOURNAL ARTICLE AU - Leonidas-Romanos Davranoglou AU - Beth Mortimer AU - Christian Matthias SchlepĆ¼tz AU - Graham K. Taylor TI - Ultra-fast micro-CT of an unrestrained live insect AID - 10.1101/2023.03.03.531017 DP - 2023 Jan 01 TA - bioRxiv PG - 2023.03.03.531017 4099 - http://biorxiv.org/content/early/2023/03/06/2023.03.03.531017.short 4100 - http://biorxiv.org/content/early/2023/03/06/2023.03.03.531017.full AB - Micro-CT has revolutionized functional morphology by enabling volumetric reconstruction of biological specimens at micrometre scales, but its accuracy is compromised by fixation artefacts. State-of-the-art in vivo imaging avoids this, but still requires subjects to be tethered, anaesthetized, or stained. Here we use ultra-fast synchrotron-based micro-CT to produce the first 3D scan of an unrestrained living organism at micrometre resolution, demonstrating the potential of this method in physiology, behaviour, and biomechanics.Competing Interest StatementThe authors have declared no competing interest.