RT Journal Article
SR Electronic
T1 The MYO1B and MYO5B motor proteins and the SNX27 sorting nexin regulate membrane mucin MUC17 trafficking in enterocytes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.03.06.530313
DO 10.1101/2023.03.06.530313
A1 Sofia Jäverfelt
A1 Gustaf Hellsén
A1 Izumi Kaji
A1 James R. Goldenring
A1 Thaher Pelaseyed
YR 2023
UL http://biorxiv.org/content/early/2023/03/07/2023.03.06.530313.abstract
AB A dense glycocalyx, composed of the megaDalton-sized membrane mucin MUC17, coats the microvilli in the apical brush border of transporting intestinal epithelial cells, called enterocytes. The establishment of the MUC17-based glycocalyx in the mouse small intestine occurs at the critical suckling-weaning transition. The enterocytic glycocalyx extends 1 µm into the intestinal lumen and prevents the gut bacteria from directly attaching to the enterocytes. To date, the mechanism behind apical targeting of MUC17 to the brush border remains unknown. Here, we show that the actin-based motor proteins MYO1B and MYO5B, and the sorting nexin SNX27 regulate the intracellular trafficking of MUC17 in enterocytes. We demonstrate that MUC17 turnover at the brush border is slow and controlled by MYO1B and SNX27. Furthermore, we report that MYO1B regulates MUC17 protein levels in enterocytes, whereas MYO5B specifically governs MUC17 levels at the brush border. Together, our results extend our understanding of the intracellular trafficking of membrane mucins and provide mechanistic insights into how defective trafficking pathways render enterocytes sensitive to bacterial invasion.Competing Interest StatementThe authors have declared no competing interest.