RT Journal Article
SR Electronic
T1 Single-Cell Map of Dynamic Multicellular Ecosystem of Radiation-Induced Intestinal Injury
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.03.06.531402
DO 10.1101/2023.03.06.531402
A1 Hua Yan
A1 Hao Lu
A1 Yuan Xing
A1 Yumeng Ye
A1 Siao Jiang
A1 Luyu Ma
A1 Hongyan Zuo
A1 Yanhui Hao
A1 Chao Yu
A1 Yang Li
A1 Yiming Lu
A1 Gangqiao Zhou
YR 2023
UL http://biorxiv.org/content/early/2023/03/07/2023.03.06.531402.abstract
AB Intestine is a highly radiation-sensitive organ that could be injured during the radiotherapy for abdominal or pelvic cavity tumors. However, the dynamic change of the intestinal microenvironment related to radiation-induced intestine injury (RIII) is still unclear. Using single-cell RNA sequencing, we pictured a dynamic landscape of the intestinal microenvironment during RIII and regeneration. We showed that the multicellular ecosystem of intestine exhibited heterogeneous radiosensitivities. We revealed the distinct dynamic patterns of three subtypes of intestinal stem cells (ISCs), and the cellular trajectory analysis suggested a complex interconversion pattern among them. For the immune cells, we found that Ly6c+ monocytes can give rise to both pro-inflammatory macrophages and resident macrophages after RIII. Besides, through cellular communication analysis, we identified a positive feedback loop between the macrophages and endothelial cells, which could amplify the inflammatory response induced by radiation. Overall, our study provides a valuable single-cell map of the dynamic multicellular ecosystem during RIII and regeneration, which may facilitate the understanding of the mechanism of RIII.Competing Interest StatementThe authors have declared no competing interest.