TY - JOUR T1 - Development and Application of Home Cage Monitoring in Laboratory Mice and Rats: a Systematic Review JF - bioRxiv DO - 10.1101/2023.03.07.531465 SP - 2023.03.07.531465 AU - Pia Kahnau AU - Paul Mieske AU - Jenny Wilzopolski AU - Otto Kalliokoski AU - Silvia Mandillo AU - Sabine M. Hölter AU - Vootele Voikar AU - Adriana Amfim AU - Sylvia Badurek AU - Aleksandra Bartelik AU - Angela Caruso AU - Maša Čater AU - Elodie Ey AU - Elisabetta Golini AU - Anne Jaap AU - Dragan Hrncic AU - Anna Kiryk AU - Benjamin Lang AU - Natasa Loncarevic-Vasiljkovic AU - Hamid Meziane AU - Aurelija Radzevičienė AU - Marion Rivalan AU - Maria Luisa Scattoni AU - Nicolas Torquet AU - Julijana Trifkovic AU - Brun Ulfhake AU - Christa Thöne-Reineke AU - Kai Diederich AU - Lars Lewejohann AU - Katharina Hohlbaum Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/03/08/2023.03.07.531465.1.abstract N2 - Traditionally, in biomedical animal research, laboratory rodents are individually examined in test apparatuses outside their home cages at selected time points. However, the outcome of such tests can be influenced by the novel environment, the time of day, separation from the social group, or the presence of an experimenter. Moreover, valuable information may be missed when the animals are only monitored in short periods. These issues can be overcome by longitudinal monitoring mice and rats in their home cages. To shed light on the development of home cage monitoring (HCM) and the current state of the art, a systematic review was carried out on 521 publications retrieved through PubMed and Web of Science. Both the absolute (∼ ×26) and relative (∼ ×7) number of HCM-related publications increased from 1974 to 2020. In both mice and rats, there was a clear bias towards males and individually housed animals, but during the past decade (2011–2020), an increasing number of studies used both sexes and group housing. More than 70 % of the studies did not involve a disease model, but the percentage of studies using disease models increased since the 2000s. In most studies, animals were kept for short (up to 4 weeks) length periods in the HCM systems; intermediate length periods (4–12 weeks) increased in frequency in the years between 2011 and 2020. Before the 2000s, HCM techniques were predominantly applied for less than 12 hours, while 24-hour measurements have been more frequently since the 2000s. The systematic review demonstrated that manual monitoring is decreasing but still relevant. Until (and including) the 1990s, most techniques were applied manually but have been progressively replaced by automation since the 2000s. Independent of the publication year, the main behavioral parameters measured were locomotor activity, feeding, and social behaviors; the main physiological parameters were heart rate and electrocardiography. External appearance-related parameters were rarely examined in the home cages. Due to technological progress and application of artificial intelligence, more refined and detailed behavioral parameters could be investigated in the home cage in recent times.Over the period covered in this study, techniques for HCM of mice and rats has improved considerably. This development is ongoing and further progress and validation of HCM systems will extend the applications to allow for continuous, longitudinal, non-invasive monitoring of an increasing range of parameters in group-housed small rodents in their home cages.Competing Interest StatementThe authors have declared no competing interest. ER -