TY - JOUR T1 - Lights, Camera, Mirrors, Action! Toolbox for 3D Analysis of High-rate Maneuvers Using a Single Camera and Planar Mirrors JF - bioRxiv DO - 10.1101/2023.03.14.532636 SP - 2023.03.14.532636 AU - Wajahat Hussain AU - Mahum Naveed AU - Asad Khan AU - Taimoor Hasan Khan AU - Muhammad Latif Anjum AU - Shahzad Rasool AU - Adnan Maqsood Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/03/15/2023.03.14.532636.abstract N2 - The advent of high-speed cameras being made available for commercial use has facilitated the discovery of intriguing animal behaviors. However, analyzing animal kinematics in three dimensions necessitates using several high-speed cameras, which are specialized equipment and come at a significant expense. In this study, we utilize a conventional methodology of capturing synchronized multiple views using a solitary camera and planar mirrors. We introduce a user-friendly software package that facilitates the calibration of this economical setup and enables the three-dimensional (3D) analysis of high-rate maneuvers. Within ∼ 15 minutes, a single high-speed camera is calibrated and can be used to acquire 3D data. We accompany the toolbox with a detailed user guide and video tutorial (https://tinyurl.com/yckm9y6b) for ease of use. We accurately reconstruct the wing beats of multiple flies (dragonfly, butterfly).Competing Interest StatementThe authors have declared no competing interest. ER -