TY - JOUR T1 - StretchfMRI: a new technique to quantify the contribution of the reticular formation to long-latency responses via fMRI JF - bioRxiv DO - 10.1101/582692 SP - 582692 AU - Andrea Zonnino AU - Andria J. Farrens AU - David Ress AU - Fabrizio Sergi Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/03/20/582692.abstract N2 - Increased reticulospinal (RS) function has been observed to cause both positive and negative outcomes in the recovery of motor function after corticospinal lesions such as stroke. Current knowledge of RF function is limited by the lack of accurate, noninvasive methods for measuring RS function. Recent studies suggest that the RS tract may be involved in processing and generating Long Latency Responses (LLRs). LLRs, elicited by applying precisely controlled perturbations, can act as a reliable stimulus to measure LLR-related brainstem function using fMRI with high signal-to-noise ratio.In this paper, we present StretchfMRI, a new technique that enables simultaneous recording of neural and muscular activity during motor responses conditioned by velocity-controlled robotic perturbations, which allows for direct investigation of the neural correlates of LLRs using fMRI.Via preliminary validation experiments, we demonstrate that our technique can reliably elicit and identify LLRs in two wrist muscles–FCR and ECU. Moreover, via a single-subject pilot experiment, we show that the occurrence of an LLR in a flexor and extensor muscle modulates neural activity in distinct regions of the brainstem. The observed somatotopic organization is in agreement with the double reciprocal model of RS function observed in animal models, in which the right medullary and left pontine reticular formation are responsible for control of the motor activity in flexors and extensors, respectively. ER -