PT  - JOURNAL ARTICLE
AU  - Markus Kilisch
AU  - Maja Gere-Becker
AU  - Liane Wüstefeld
AU  - Christel Bonnas
AU  - Alexander Crauel
AU  - Maja Mechmershausen
AU  - Henrik Martens
AU  - Hansjörg Götzke
AU  - Felipe Opazo
AU  - Steffen Frey
TI  - Simple and highly efficient detection of PSD95 using a nanobody and its recombinant minibody derivative
AID  - 10.1101/2023.03.16.532938
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.03.16.532938
4099  - http://biorxiv.org/content/early/2023/03/17/2023.03.16.532938.short
4100  - http://biorxiv.org/content/early/2023/03/17/2023.03.16.532938.full
AB  - The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has been implicated with several neurological disorders, making it an attractive target to develop strategies able to monitor PSD95 accurately for diagnostics and therapeutics. This study characterizes a novel camelid single-domain antibody (nanobody) that binds strongly and with high specificity to rat, mouse and human PSD95. This nanobody allows for a more precise detection and better quantification of PSD95 in various biological samples. We expect that the flexibility and unique performance of this thoroughly characterized affinity tool will help to further understand the role of PSD95 in normal and diseased neuronal synapses.Competing Interest StatementAuthors of this manuscript are employees or shareholders of NanoTag Biotechnologies GmbH or Synaptic Systems GmbH.