RT Journal Article
SR Electronic
T1 Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.03.17.484738
DO 10.1101/2022.03.17.484738
A1 Jonathan Michael Dragwidge
A1 Yanning Wang
A1 Lysiane Brocard
A1 Andreas De Meyer
A1 Roman Hudeček
A1 Dominique Eeckhout
A1 Peter Grones
A1 Matthieu Buridan
A1 Clément Chambaud
A1 Přemysl Pejchar
A1 Martin Potocký
A1 Joanna Winkler
A1 Michael Vandorpe
A1 Nelson Serre
A1 Matyáš Fendrych
A1 Amelie Bernard
A1 Geert De Jaeger
A1 Roman Pleskot
A1 Xiaofeng Fang
A1 Daniël Van Damme
YR 2023
UL http://biorxiv.org/content/early/2023/03/18/2022.03.17.484738.abstract
AB Clathrin-mediated endocytosis (CME) is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form membrane-bound vesicles. In plants, the evolutionarily ancient TSET/TPLATE complex (TPC) plays an essential, but not well-defined role in CME. Here, we show that two highly disordered TPC subunits, AtEH1 and AtEH2 function as scaffolds to drive biomolecular condensation of the complex. These condensates specifically nucleate on the plasma membrane through interactions with anionic phospholipids, and facilitate the dynamic recruitment and assembly of clathrin, early-, and late-stage endocytic accessory proteins. Importantly, clathrin forms ordered assemblies within the condensate environment. Biomolecular condensation therefore acts to promote dynamic protein assemblies throughout clathrin-mediated endocytosis. Furthermore, the disordered region sequence properties of AtEH1 regulate the material properties of the endocytic condensates in vivo and alteration of these material properties influences endocytosis dynamics, and consequently plant adaptive growth.HighlightsAtEH subunits are endocytic scaffolds which drive condensation of the TPCAtEH1 condensates nucleate on the plasma membrane via lipid interactionsCondensation of AtEH1/TPC facilitates clathrin re-arrangement and assemblyAtEH1 IDR1 composition controls condensate properties to regulate endocytosisCompeting Interest StatementThe authors have declared no competing interest.