PT - JOURNAL ARTICLE AU - Mihai, Ionut Sebastian AU - Rosenbaum, William AU - Dernstedt, Andy AU - Gröning, Remigius AU - Trygg, Johan AU - Forsell, Mattias AU - Henriksson, Johan TI - Telomemore enables single-cell analysis of cell cycle and chromatin condensation AID - 10.1101/2023.03.19.533267 DP - 2023 Jan 01 TA - bioRxiv PG - 2023.03.19.533267 4099 - http://biorxiv.org/content/early/2023/03/23/2023.03.19.533267.short 4100 - http://biorxiv.org/content/early/2023/03/23/2023.03.19.533267.full AB - It is now easy to perform multiome single-cell analysis, including both RNA and ATAC readouts from the same cell. This enables a closer linkage between the two types of modalities, but it remains an open question what more information can be extracted from this type of data. ATAC-seq is normally only used to assay transcription factor binding to open regions. By reanalyzing several large datasets, and generating an atlas of B cells, we show that telomere accessibility can better pinpoint processes related to cell cycle and chromatin condensation. We provide Telomemore, a tool that can extract telomeric reads, and give examples of new findings it enables. Our new findings will aid in the annotation and analysis of single-cell ATAC or multiome datasets.Competing Interest StatementJ.T. is employed at Sartorius. I.S.M is employed at Umea university but partially funded by Sartorius. Other authors declare no conflict of interest.