TY - JOUR T1 - Distinct dystrophin and Wnt/Ror-dependent pathways establish planar-polarized membrane compartments in <em>C. elegans</em> muscles JF - bioRxiv DO - 10.1101/2023.03.28.534519 SP - 2023.03.28.534519 AU - Alice Peysson AU - Noura Zariohi AU - Marie Gendrel AU - Amandine Chambert-Loir AU - Noémie Frébault AU - Olga Andrini AU - Thomas Boulin Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/03/28/2023.03.28.534519.abstract N2 - The plasma membrane of excitable cells is highly structured and molecular scaffolds recruit proteins to specific membrane compartments. Here, we show that potassium channels and proteins belonging to the dystrophin-associated protein complex define multiple types of planar-polarized membrane compartments at the surface of C. elegans muscle cells. Surprisingly, conserved planar cell polarity proteins are not required for this process. However, we implicate a Wnt signaling module involving the Wnt ligand EGL-20, the Wnt receptor CAM-1, and the intracellular effector DSH-1/disheveled in the formation of this cell polarity pattern. Moreover, using time-resolved and tissue-specific protein degradation, we demonstrate that muscle cell polarity is a dynamic state, requiring continued presence of DSH-1 throughout post-embryonic life. Our results reveal the intricate, highly reproducible, and entirely unsuspected complexity of the worm’s sarcolemma. This novel case of planar cell polarity in a tractable genetic model organism may provide valuable insight into the molecular and cellular mechanisms that regulate cellular organization, allowing specific functions to be compartmentalized within distinct plasma membrane domains.Competing Interest StatementThe authors have declared no competing interest. ER -