RT Journal Article
SR Electronic
T1 Probing inter-areal computations with a cellular resolution two-photon holographic mesoscope
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.03.02.530875
DO 10.1101/2023.03.02.530875
A1 Abdeladim, Lamiae
A1 Shin, Hyeyoung
A1 Jagadisan, Uday K.
A1 Ogando, Mora B.
A1 Adesnik, Hillel
YR 2023
UL http://biorxiv.org/content/early/2023/04/10/2023.03.02.530875.abstract
AB Brain computation depends on intricately connected yet highly distributed neural networks. Due to the absence of the requisite technologies, causally testing fundamental hypotheses on the nature of inter-areal processing have remained largely out-of-each. Here we developed the first two photon holographic mesoscope, a system capable of simultaneously reading and writing neural activity patterns with single cell resolution across large regions of the brain. We demonstrate the precise photo-activation of spatial and temporal sequences of neurons in one brain area while reading out the downstream effect in several other regions. Investigators can use this new platform to understand feed-forward and feed-back processing in distributed neural circuits with single cell precision for the first time.Competing Interest StatementThe authors have declared no competing interest.