RT Journal Article SR Electronic T1 Single-cell transcriptomics of malaria parasites JF bioRxiv FD Cold Spring Harbor Laboratory SP 105015 DO 10.1101/105015 A1 Reid, Adam J. A1 Talman, Arthur M. A1 Bennett, Hayley M. A1 Gomes, Ana R. A1 Sanders, Mandy J. A1 Illingworth, Christopher J. R. A1 Billker, Oliver A1 Berriman, Matthew A1 Lawniczak, Mara K. N. YR 2017 UL http://biorxiv.org/content/early/2017/02/10/105015.abstract AB Single-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations, but has not yet been applied to single cell organisms. Here, we established a method to successfully investigate transcriptional variation across individual malaria parasites. We discover an unexpected, discontinuous program of transcription during asexual growth previously masked by bulk analyses, and uncover novel variation among sexual stage parasites in their expression of gene families important in host-parasite interactions.