RT Journal Article
SR Electronic
T1 Single-cell transcriptomics of malaria parasites
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 105015
DO 10.1101/105015
A1 Adam J. Reid
A1 Arthur M. Talman
A1 Hayley M. Bennett
A1 Ana R. Gomes
A1 Mandy J. Sanders
A1 Christopher J. R. Illingworth
A1 Oliver Billker
A1 Matthew Berriman
A1 Mara K. N. Lawniczak
YR 2017
UL http://biorxiv.org/content/early/2017/02/10/105015.abstract
AB Single-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations, but has not yet been applied to single cell organisms. Here, we established a method to successfully investigate transcriptional variation across individual malaria parasites. We discover an unexpected, discontinuous program of transcription during asexual growth previously masked by bulk analyses, and uncover novel variation among sexual stage parasites in their expression of gene families important in host-parasite interactions.