RT Journal Article
SR Electronic
T1 Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.04.17.537163
DO 10.1101/2023.04.17.537163
A1 Stout, Andrew J.
A1 Zhang, Xiaoli
A1 Letcher, Sophia M.
A1 Rittenberg, Miriam L.
A1 Shub, Michelle
A1 Chai, Kristin M.
A1 Kaul, Maya
A1 Kaplan, David L.
YR 2023
UL http://biorxiv.org/content/early/2023/04/17/2023.04.17.537163.abstract
AB Cultured meat is a promising technology that faces substantial cost barriers which are currently driven largely by the price of media components. Growth factors such as fibroblast growth factor 2 (FGF2) drive the cost of serum-free media for relevant cells including muscle satellite cells. Here, we engineered immortalized bovine satellite cells (iBSCs) for inducible expression of FGF2 and/or mutated RasG12V in order to overcome media growth factor requirements through autocrine signaling. Engineered cells were able to proliferate over multiple passages in FGF2-free medium, thereby eliminating the need for this costly component. Additionally, cells maintained their myogenicity, albeit with reduced differentiation capacity. Ultimately, this offers a proof-of-principle for lower-cost cultured meat production through cell line engineering.Competing Interest StatementThe authors have declared no competing interest.