PT  - JOURNAL ARTICLE
AU  - Tatsuya Nobori
AU  - Alexander Monell
AU  - Travis A. Lee
AU  - Jingtian Zhou
AU  - Joseph Nery
AU  - Joseph R. Ecker
TI  - Time-resolved single-cell and spatial gene regulatory atlas of plants under pathogen attack
AID  - 10.1101/2023.04.10.536170
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.04.10.536170
4099  - http://biorxiv.org/content/early/2023/04/24/2023.04.10.536170.short
4100  - http://biorxiv.org/content/early/2023/04/24/2023.04.10.536170.full
AB  - Plant leaf intercellular space provides a nutrient-rich and heterogeneous niche for microbes that critically impacts plant health. However, how individual plant cells respond to heterogeneous microbial colonization remains largely elusive. Here, by time-resolved simultaneous single-cell transcriptome and epigenome profiling of plants (Arabidopsis thaliana) infected by virulent and avirulent bacterial pathogens (Pseudomonas syringae), we present cell atlases with gene regulatory logic involving transcription factors, putative cis-regulatory elements, and target genes associated with disease and immunity. We also identify previously uncharacterized cell populations with distinct immune gene expression within major developmental cell types. Furthermore, we employ time-resolved spatial transcriptomics to reveal spatial heterogeneity of plant immune responses linked to pathogen distribution. Integrating our single-cell multiomics and spatial omics data enables spatiotemporal mapping of defense gene regulatory logic with pathogen cells. Our study provides a molecularly-defined spatiotemporal map of plant-microbe interaction at the single-cell resolution.Competing Interest StatementThe authors have declared no competing interest.