RT Journal Article
SR Electronic
T1 Harnessing sleep/wake state tracking by olfactory bulb oscillations to perform fully brain-based sleep scoring in mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 109033
DO 10.1101/109033
A1 Sophie Bagur
A1 Marie Masako Lacroix
A1 Gaëtan de Lavilléon
A1 Julie M Lefort
A1 Hélène Geoffroy
A1 Karim Benchenane
YR 2017
UL http://biorxiv.org/content/early/2017/02/16/109033.abstract
AB There is growing interest in sleep research and finding easily tracked neural correlates of brain states is a central challenge to the definition of sleep and wake states. Here we demonstrate using multi-site electrophysiological LFP recordings in freely moving mice that gamma power in the olfactory bulb (OB) allows for clear classification of sleep and wake. Coupled with hippocampal theta activity, it allows the construction of a robust and reproducible sleep scoring algorithm that relies on brain activity alone. We validate the procedure by comparison with classical methods based on muscular activity (EMG) and video tracking. Contrary to EMG, OB gamma power allows correct discrimination between sleep and immobility in ambiguous situations such as fear-related freezing. Finally, beta power in the OB is a good predictor of Rapid Eye Movement sleep. Overall, our results reveal the OB can be used as a highly reliable readout of brain states.