RT Journal Article
SR Electronic
T1 A Wireless Optoelectronic Probe Monitors Tissue Oxygenation in the Deep Brain
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.05.21.541646
DO 10.1101/2023.05.21.541646
A1 Xue Cai
A1 Haijian Zhang
A1 Penghu Wei
A1 Quanlei Liu
A1 Dawid Sheng
A1 Zhen Li
A1 Wenxin Zhao
A1 Zhongyin Ye
A1 Zhao Xue
A1 Yang Xie
A1 Yang Dai
A1 Changming Wang
A1 Yuqi Wang
A1 Xin Fu
A1 Bozhen Zhang
A1 Lan Yin
A1 Hongshang Peng
A1 He Ding
A1 Guoguang Zhao
A1 Xing Sheng
YR 2023
UL http://biorxiv.org/content/early/2023/05/23/2023.05.21.541646.abstract
AB Real-time detection of tissue oxygenation in the nervous system is crucial for neuroscience exploration and clinical diagnostics. Compared to blood oxygenation, the partial pressure of oxygen in brain tissue (PbtO2) possesses more direct relevance to local neural activities and metabolic conditions. In this paper, we present an implantable optoelectronic probe that wirelessly and continuously monitors PbtO2 signals in the deep brain of living animals. The thin-film, microscale implant integrates a light-emitting diode and a photodetector coated with oxygen sensitive dyes. Powered by a battery or an inductive coil, a miniaturized circuit is capable of recording and wirelessly transmitting PbtO2 signals, which allows for simultaneous monitoring of PbtO2 levels in multiple freely moving rodents. The wireless micro-probe captures cerebral hypoxia states of mice in various scenarios, including altered inspired oxygen concentration, acute ischemia. Particularly, in mouse models with seizures, the micro-probe associates temporal PbtO2 variations in multiple brain regions with electrical stimulations imposed in the hippocampus. These materials and device strategies overcome the limits of existing oxygen sensing approaches and provide important insights into neurometabolic coupling.Competing Interest StatementThe authors have declared no competing interest.