PT  - JOURNAL ARTICLE
AU  - Ben-Jie Li
AU  - Kun-Kun Wang
AU  - Ye Yu
AU  - Jia-Qi Wei
AU  - Jian Zhu
AU  - Jia-Li Wang
AU  - Fei Lin
AU  - Han-Hong Xu
TI  - Bacterial <em>PxRdl2</em> dsRNA increased the insecticidal activities of GABAR-targeting compounds against <em>Plutella xylostella</em>
AID  - 10.1101/2021.03.24.436736
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2021.03.24.436736
4099  - http://biorxiv.org/content/early/2023/05/31/2021.03.24.436736.short
4100  - http://biorxiv.org/content/early/2023/05/31/2021.03.24.436736.full
AB  - The utilization of RNA interference (RNAi) for pest management has garnered global interest. The bioassay results suggested the knockout of PxRdl2 significantly increased the insecticidal activities of the γ-aminobutyric acid receptor (GABAR) targeting compounds (Fipronil, two pyrazoloquinazolines and two isoxazolines), thereby presenting a viable target gene for RNAi-mediated pest control. Consequently, we suggest enhancing the insecticidal activities of GABAR-targeting compounds by knockdown the transcript level of PxRdl2. Furthermore, PxRdl2 dsRNA was expressed in HT115 Escherichia coli to reduce costs and protect dsRNA against degradation. In comparison to in vitro synthesized dsRNA, the recombinant bacteria (ds-B) exhibited superior interference efficiencies and greater stability when exposed to UV irradiation. Collectively, our results provide a new strategy of insecticide spray which combined synergistically with insecticidal activities by suppressing PxRdl2 using ds-B, and may be beneficial for reducing the usage of insecticide and slowing pest resistance.Competing Interest StatementThe authors have declared no competing interest.