RT Journal Article
SR Electronic
T1 Bacterial PxRdl2 dsRNA increased the insecticidal activities of GABAR-targeting compounds against Plutella xylostella
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.03.24.436736
DO 10.1101/2021.03.24.436736
A1 Ben-Jie Li
A1 Kun-Kun Wang
A1 Ye Yu
A1 Jia-Qi Wei
A1 Jian Zhu
A1 Jia-Li Wang
A1 Fei Lin
A1 Han-Hong Xu
YR 2023
UL http://biorxiv.org/content/early/2023/05/31/2021.03.24.436736.abstract
AB The utilization of RNA interference (RNAi) for pest management has garnered global interest. The bioassay results suggested the knockout of PxRdl2 significantly increased the insecticidal activities of the γ-aminobutyric acid receptor (GABAR) targeting compounds (Fipronil, two pyrazoloquinazolines and two isoxazolines), thereby presenting a viable target gene for RNAi-mediated pest control. Consequently, we suggest enhancing the insecticidal activities of GABAR-targeting compounds by knockdown the transcript level of PxRdl2. Furthermore, PxRdl2 dsRNA was expressed in HT115 Escherichia coli to reduce costs and protect dsRNA against degradation. In comparison to in vitro synthesized dsRNA, the recombinant bacteria (ds-B) exhibited superior interference efficiencies and greater stability when exposed to UV irradiation. Collectively, our results provide a new strategy of insecticide spray which combined synergistically with insecticidal activities by suppressing PxRdl2 using ds-B, and may be beneficial for reducing the usage of insecticide and slowing pest resistance.Competing Interest StatementThe authors have declared no competing interest.