PT  - JOURNAL ARTICLE
AU  - Jeong, Hyun-Hwan
AU  - Kim, Seon Young
AU  - Rosseaux, Maxime WC
AU  - Zoghbi, Huda Y
AU  - Liu, Zhandong
TI  - SAVE: A secure cloud-based pipeline for CRISPR pooled screen deconvolution
AID  - 10.1101/110262
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 110262
4099  - http://biorxiv.org/content/early/2017/02/21/110262.short
4100  - http://biorxiv.org/content/early/2017/02/21/110262.full
AB  - We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, termed SAVE for Screening Analysis Visual Explorer, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, SAVE serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data. SAVE is available at http://save.nrihub.org.