PT - JOURNAL ARTICLE AU - H’ng, Chee Ho AU - Amarasinghe, Shanika L. AU - Zhang, Boya AU - Chang, Hojin AU - Powell, David AU - Rosello-Diez, Alberto TI - Compensatory growth and recovery of tissue cytoarchitecture after transient cartilage-specific cell death in foetal mouse limbs AID - 10.1101/2023.06.20.545035 DP - 2023 Jan 01 TA - bioRxiv PG - 2023.06.20.545035 4099 - http://biorxiv.org/content/early/2023/06/20/2023.06.20.545035.short 4100 - http://biorxiv.org/content/early/2023/06/20/2023.06.20.545035.full AB - A major question in developmental and regenerative biology is how organ size is controlled by progenitor cells. For example, while limb bones exhibit catch-up growth (recovery of a normal growth trajectory after transient developmental perturbation), it is unclear how this emerges from the behaviour of chondroprogenitors, the cells sustaining the cartilage anlagen that are progressively replaced by bone. Here we show that transient sparse cell death in the mouse foetal cartilage was repaired postnatally, via a two-step process. During injury, progression of chondroprogenitors towards more differentiated states was delayed, leading to altered cartilage cytoarchitecture and impaired bone growth. Then, once cell death was over, chondroprogenitor differentiation was accelerated and cartilage structure recovered, including partial rescue of bone growth. At the molecular level, ectopic activation of mTORC1 correlated with, and was necessary for, part of the recovery, revealing a specific candidate to be explored during normal growth and in future therapies.Competing Interest StatementThe authors have declared no competing interest.