PT  - JOURNAL ARTICLE
AU  - Yu, Fang
AU  - Courjaret, Raphael
AU  - Elmi, Asha
AU  - Hammad, Ayat
AU  - Fisher, Melanie
AU  - Terasaki, Mark
AU  - Machaca, Khaled
TI  - ER-Mitochondria Contact Sites expand during mitosis
AID  - 10.1101/2023.06.22.546089
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.06.22.546089
4099  - http://biorxiv.org/content/early/2023/06/22/2023.06.22.546089.short
4100  - http://biorxiv.org/content/early/2023/06/22/2023.06.22.546089.full
AB  - Membrane contact sites between various organelles define specialized spatially defined signaling hubs, they are of great interest to better understand inter-organelle communication and its implications on cellular physiology. ER-mitochondria contact sites (ERMCS) are one of the best studied and mediate Ca2+ signaling that regulates mitochondrial bioenergetics. However, little is known about ERMCS during mitosis. Here we show that ERMCS expand during mitosis using transmission electron microscopy, serial EM coupled to 3D reconstruction, and ERMCS markers. ERMCS expansion in mitosis is functionally significant as it is associated with enhanced Ca2+ coupling between the ER and mitochondria resulting in heightened activation of mitochondrial dehydrogenases. Our data suggest that ERMCS remodeling in mitosis is important to meet the increased energy needs during cell division.Competing Interest StatementThe authors have declared no competing interest.