PT - JOURNAL ARTICLE AU - Hamada, Yuta AU - Kitamura, Akira TI - Light-induced condensates show accumulation-prone and less dynamic properties in the nucleus compared to the cytoplasm AID - 10.1101/2023.06.07.544154 DP - 2023 Jan 01 TA - bioRxiv PG - 2023.06.07.544154 4099 - http://biorxiv.org/content/early/2023/06/28/2023.06.07.544154.short 4100 - http://biorxiv.org/content/early/2023/06/28/2023.06.07.544154.full AB - Biomolecular condensates, including membraneless organelles, are ubiquitously observed in subcellular compartments. However, the accumulation and dynamic properties of arbitrarily induced condensates remain elusive. Here, we show the size, amount, and dynamic properties of subcellular condensates using various fluorescence spectroscopic imaging analyses. Spatial image correlation spectroscopy showed that the size of blue-light-induced condensates of cryptochrome 2-derived oligomerization tag (CRY2olig) tagged with a red fluorescent protein in the nucleus was not different from that in the cytoplasm. Fluorescence intensity measurements showed that the condensates in the nucleus were more prone to accumulation than those in the cytoplasm. Single-particle tracking analysis showed that the condensates in the nucleus are predisposed to be stationary dynamics compared to those in the cytoplasm. Therefore, the subcellular compartment may, in part, affect the characteristics of self-recruitment of biomolecules in the condensates and their movement property.Competing Interest StatementThe authors have declared no competing interest.