RT Journal Article
SR Electronic
T1 Light-induced condensates show accumulation-prone and less dynamic properties in the nucleus compared to the cytoplasm
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.06.07.544154
DO 10.1101/2023.06.07.544154
A1 Hamada, Yuta
A1 Kitamura, Akira
YR 2023
UL http://biorxiv.org/content/early/2023/06/28/2023.06.07.544154.abstract
AB Biomolecular condensates, including membraneless organelles, are ubiquitously observed in subcellular compartments. However, the accumulation and dynamic properties of arbitrarily induced condensates remain elusive. Here, we show the size, amount, and dynamic properties of subcellular condensates using various fluorescence spectroscopic imaging analyses. Spatial image correlation spectroscopy showed that the size of blue-light-induced condensates of cryptochrome 2-derived oligomerization tag (CRY2olig) tagged with a red fluorescent protein in the nucleus was not different from that in the cytoplasm. Fluorescence intensity measurements showed that the condensates in the nucleus were more prone to accumulation than those in the cytoplasm. Single-particle tracking analysis showed that the condensates in the nucleus are predisposed to be stationary dynamics compared to those in the cytoplasm. Therefore, the subcellular compartment may, in part, affect the characteristics of self-recruitment of biomolecules in the condensates and their movement property.Competing Interest StatementThe authors have declared no competing interest.