PT  - JOURNAL ARTICLE
AU  - Miles, Lee B
AU  - Calcinotto, Vanessa
AU  - Sonntag, Carmen
AU  - Lee, Clara
AU  - Bryson-Richardson, Robert J
TI  - CRIMP: A CRISPR/Cas9 Insertional Mutagenesis Protocol and the CRIMP Toolkit
AID  - 10.1101/2023.07.13.548929
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.07.13.548929
4099  - http://biorxiv.org/content/early/2023/07/14/2023.07.13.548929.short
4100  - http://biorxiv.org/content/early/2023/07/14/2023.07.13.548929.full
AB  - We developed a highly efficient targeted insertional mutagenesis system, CRIMP, and an associated plasmid toolkit, CRIMPkit, that disrupts native gene expression by inducing complete transcriptional termination to produce null mutant alleles without inducing genetic compensation. The CRIMPkit contains over 30 ready-to-use plasmid vectors allowing easy and complete mutagenesis of any gene in any reading frame without requiring custom sequences, modification, or subcloning, and also provides a fluorescent readout of successfully mutagenised fish.Competing Interest StatementThe authors have declared no competing interest.