RT Journal Article
SR Electronic
T1 CRIMP: A CRISPR/Cas9 Insertional Mutagenesis Protocol and the CRIMP Toolkit
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.07.13.548929
DO 10.1101/2023.07.13.548929
A1 Miles, Lee B
A1 Calcinotto, Vanessa
A1 Sonntag, Carmen
A1 Lee, Clara
A1 Bryson-Richardson, Robert J
YR 2023
UL http://biorxiv.org/content/early/2023/07/14/2023.07.13.548929.abstract
AB We developed a highly efficient targeted insertional mutagenesis system, CRIMP, and an associated plasmid toolkit, CRIMPkit, that disrupts native gene expression by inducing complete transcriptional termination to produce null mutant alleles without inducing genetic compensation. The CRIMPkit contains over 30 ready-to-use plasmid vectors allowing easy and complete mutagenesis of any gene in any reading frame without requiring custom sequences, modification, or subcloning, and also provides a fluorescent readout of successfully mutagenised fish.Competing Interest StatementThe authors have declared no competing interest.