PT  - JOURNAL ARTICLE
AU  - Francesca Billwiller
AU  - Laura Castillo
AU  - Heba Elseedy
AU  - Anton Ivanovich Ivanov
AU  - Jennyfer Scapula
AU  - Antoine Ghestem
AU  - Julien Carponcy
AU  - Paul Antoine Libourel
AU  - Hélène Bras
AU  - Nabila ElSayed Abdelmeguid
AU  - Esther Krook-Magnuson
AU  - Ivan Soltesz
AU  - Christophe Bernard
AU  - Pierre-Hervé Luppi
AU  - Monique Esclapez
TI  - The GABA-Glutamate supramammillary–dorsal Dentate Gyrus pathway controls theta and gamma oscillations in the DG during paradoxical sleep
AID  - 10.1101/584862
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 584862
4099  - http://biorxiv.org/content/early/2019/03/21/584862.short
4100  - http://biorxiv.org/content/early/2019/03/21/584862.full
AB  - Several studies suggest that neurons from the lateral region of the SuM (SuML) innervating the dorsal dentate gyrus (DG) display a dual GABAergic and glutamatergic transmission and are specifically activated during paradoxical (REM) sleep (PS). The objective of the present study is to fully characterize the anatomical, neurochemical and electrophysiological properties of the SuML-DG projection neurons and to determine how they control DG oscillations and neuronal activation during PS and other vigilance states. For this purpose, we combine structural connectivity techniques using neurotropic viral vectors (rabies virus, AAV), neurochemical anatomy (immunohistochemistry, in situ hybridization) and imaging (light, electron and confocal microscopy) with in vitro (patch clamp) and in vivo (LFP, EEG) optogenetic and electrophysiological recordings performed in transgenic VGLUT2-cre mice. At the cellular level, we show that the SuML-DG neurons co-releases GABA and glutamate on dentate granule cells and increase the activity of a subset of DG granule cells. At the network level, we show that the activation of the SuML-DG pathway increases theta power and frequency during PS as well as gamma power during PS and waking in the DG. At the behavioral level, we show that the activation of this pathway does not change animal behavior during PS, induces awakening during slow wave sleep and increases motor and exploratory activity during waking. These results suggest that the SuML-DG pathway is capable of supporting the increase of theta and gamma power in the DG observed during PS and plays an important modulatory role of DG network activity during this state.