PT  - JOURNAL ARTICLE
AU  - Sven A. Sewitz
AU  - Zahra Fahmi
AU  - Latifa Aljebali
AU  - Jeremy Bancroft
AU  - Otávio J. B. Brustolini
AU  - Hicham Saad
AU  - Isabelle Goiffon
AU  - Csilla Vamai
AU  - Steven Wingett
AU  - Hua Wong
AU  - Biola-Maria Javierre
AU  - Stefan Schoenfelder
AU  - Simon Andrews
AU  - Stephen G. Oliver
AU  - Peter Fraser
AU  - Kerstin Bystricky
AU  - Karen Lipkow
TI  - Heterogeneous chromatin mobility derived from chromatin states is a determinant of genome organisation in <em>S. cerevisiae</em>
AID  - 10.1101/106344
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 106344
4099  - http://biorxiv.org/content/early/2017/03/02/106344.short
4100  - http://biorxiv.org/content/early/2017/03/02/106344.full
AB  - Spatial organisation of the genome is essential for the activation of correct gene expression profiles, but the mechanisms that shape three-dimensional genome organisation in eukaryotes are still far from understood. Here, we develop a new approach, combining bioinformatic determination of chromatin states, dynamic polymer modelling of genome structure, quantitative microscopy and Hi-C to demonstrate that differential mobility of yeast chromosome segments leads to self-organisation of the genome in three dimensions. More than forty percent of chromatin-associated proteins display a poised distribution and coordinated relocations. They are distributed heterogeneously along the chromosome, and by simulating the dynamics of this heteropolymer, we observe structural features that match our experimental results. Further, we show that this mechanism directly contributes to the directed relocalisation of active genes to the nuclear periphery.One Sentence Summary Unequal protein occupancy and chromosome segment mobility drive 3D organisation of the genome.