TY - JOUR T1 - Integrin-dependent YAP signaling requires LAMTOR1 mediated delivery of Src to the plasma membrane JF - bioRxiv DO - 10.1101/585349 SP - 585349 AU - Marc R. Block AU - Molly Brunner AU - Théo Ziegelmeyer AU - Dominique Lallemand AU - Mylène Pezet AU - Genevieve Chevalier AU - Philippe Rondé AU - Bernhard Wehrle-Haller AU - Daniel Bouvard Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/03/23/585349.abstract N2 - YAP signaling has emerged as an important signaling pathway involved in several normal and pathological processes. While main upstream effectors regulating its activity have been extensively studied, the interplay with other cellular processes has been far less analyzed. Here, we identified the LAMTOR complex as a new important regulator of YAP signaling. We uncovered that p18/LAMTOR1 is required for the recycling of Src on late endosomes to the cell periphery, and consequently to activate a signaling cascade that eventually controls YAP nuclear shuttling. Moreover, p18/LAMTOR1 positives late endosomes distribution is controlled by β1 integrins, extracellular matrix stiffness and cell contractility. This likely relies on the targeting of microtubules to β1 positive focal adhesion via ILK. Altogether our findings identify the late endosomal recycling pathway as a major regulator of YAP. ER -