RT Journal Article
SR Electronic
T1 Integrin-dependent YAP signaling requires LAMTOR1 mediated delivery of Src to the plasma membrane
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 585349
DO 10.1101/585349
A1 Marc R. Block
A1 Molly Brunner
A1 Théo Ziegelmeyer
A1 Dominique Lallemand
A1 Mylène Pezet
A1 Genevieve Chevalier
A1 Philippe Rondé
A1 Bernhard Wehrle-Haller
A1 Daniel Bouvard
YR 2019
UL http://biorxiv.org/content/early/2019/03/23/585349.abstract
AB YAP signaling has emerged as an important signaling pathway involved in several normal and pathological processes. While main upstream effectors regulating its activity have been extensively studied, the interplay with other cellular processes has been far less analyzed. Here, we identified the LAMTOR complex as a new important regulator of YAP signaling. We uncovered that p18/LAMTOR1 is required for the recycling of Src on late endosomes to the cell periphery, and consequently to activate a signaling cascade that eventually controls YAP nuclear shuttling. Moreover, p18/LAMTOR1 positives late endosomes distribution is controlled by β1 integrins, extracellular matrix stiffness and cell contractility. This likely relies on the targeting of microtubules to β1 positive focal adhesion via ILK. Altogether our findings identify the late endosomal recycling pathway as a major regulator of YAP.