PT  - JOURNAL ARTICLE
AU  - Foyt, Daniel
AU  - Brown, David
AU  - Zhou, Shuqin
AU  - Huang, Bo
TI  - HybriSeq: Probe-based Device-free Single-cell RNA Profiling
AID  - 10.1101/2023.09.27.559406
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.09.27.559406
4099  - http://biorxiv.org/content/early/2023/09/29/2023.09.27.559406.short
4100  - http://biorxiv.org/content/early/2023/09/29/2023.09.27.559406.full
AB  - We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection with multiple probes and profile RNA accessibility. The utility of HybriSeq is demonstrated in characterizing cell-to-cell heterogeneities of a panel of 95 cell-cycle-related genes and the probe-probe heterogeneity within a single transcript.Competing Interest StatementThe authors have declared no competing interest.