RT Journal Article
SR Electronic
T1 HybriSeq: Probe-based Device-free Single-cell RNA Profiling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.09.27.559406
DO 10.1101/2023.09.27.559406
A1 Foyt, Daniel
A1 Brown, David
A1 Zhou, Shuqin
A1 Huang, Bo
YR 2023
UL http://biorxiv.org/content/early/2023/09/29/2023.09.27.559406.abstract
AB We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection with multiple probes and profile RNA accessibility. The utility of HybriSeq is demonstrated in characterizing cell-to-cell heterogeneities of a panel of 95 cell-cycle-related genes and the probe-probe heterogeneity within a single transcript.Competing Interest StatementThe authors have declared no competing interest.