PT  - JOURNAL ARTICLE
AU  - Chua, Eugene YD
AU  - Alink, Lambertus M
AU  - Kopylov, Mykhailo
AU  - Johnston, Jake
AU  - Eisenstein, Fabian
AU  - de Marco, Alex
TI  - Square beams for optimal tiling in TEM
AID  - 10.1101/2023.07.29.551095
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.07.29.551095
4099  - http://biorxiv.org/content/early/2023/10/09/2023.07.29.551095.short
4100  - http://biorxiv.org/content/early/2023/10/09/2023.07.29.551095.full
AB  - Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.Competing Interest StatementThe authors have declared no competing interest.