RT Journal Article
SR Electronic
T1 Square beams for optimal tiling in TEM
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.07.29.551095
DO 10.1101/2023.07.29.551095
A1 Chua, Eugene YD
A1 Alink, Lambertus M
A1 Kopylov, Mykhailo
A1 Johnston, Jake
A1 Eisenstein, Fabian
A1 de Marco, Alex
YR 2023
UL http://biorxiv.org/content/early/2023/10/09/2023.07.29.551095.abstract
AB Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.Competing Interest StatementThe authors have declared no competing interest.