PT - JOURNAL ARTICLE AU - Hassani, Seyed A. AU - Womelsdorf, Thilo TI - Noradrenergic alpha-2a Receptor Stimulation Enhances Prediction Error Signaling in Anterior Cingulate Cortex and Striatum AID - 10.1101/2023.10.25.564052 DP - 2023 Jan 01 TA - bioRxiv PG - 2023.10.25.564052 4099 - http://biorxiv.org/content/early/2023/10/25/2023.10.25.564052.short 4100 - http://biorxiv.org/content/early/2023/10/25/2023.10.25.564052.full AB - The noradrenergic system is implicated to support behavioral flexibility by increasing exploration during periods of uncertainty and by enhancing working memory for goal-relevant stimuli. Possible sources mediating these pro-cognitive effects are α2A adrenoceptors (α2AR) in prefrontal cortex or the anterior cingulate cortex facilitating fronto-striatal learning processes. We tested this hypothesis by selectively stimulating α2ARs using Guanfacine during feature-based attentional set shifting in nonhuman primates. We found that α2A stimulation improved learning from errors and facilitates updating the target feature of an attentional set. Neural recordings in the anterior cingulate cortex (ACC), the dorsolateral prefrontal cortex (dlPFC), and the striatum showed that α2A stimulation selectively enhanced the neural representation of negative reward prediction errors in neurons of the ACC and of positive prediction errors in the striatum, but not in dlPFC. This modulation was accompanied by enhanced encoding of the feature and location of the attended target across the fronto-striatal network. Enhanced learning was paralleled by enhanced encoding of outcomes in putative fast-spiking interneurons in the ACC, dlPFC, and striatum but not in broad spiking cells, pointing to an interneuron mediated mechanism of α2AR action. These results illustrate that α2A receptors causally support the noradrenergic enhancement of updating attention sets through an enhancement of prediction error signaling in the ACC and the striatum.Competing Interest StatementThe authors have declared no competing interest.