RT Journal Article
SR Electronic
T1 Genotyping of zebrafish embryos fixed by paraformaldehyde
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 118331
DO 10.1101/118331
A1 Yang, Xiaojie
A1 Gu, Qilin
YR 2017
UL http://biorxiv.org/content/early/2017/03/19/118331.abstract
AB The rapid generation of various species and strains of zebrafish using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, several approaches for genotyping of unfixed genome-modified animals have been successfully developed, such as Surveyor assays, T7 endonuclease 1 (T7E1) assays, High Resolution Melt Analysis (HRMA) and PAGE-based genotyping approach. However, there is few published genotyping protocol for the embryos of lethal zebrafish mutants fixed by paraformaldehyde. We have designed this genotyping protocol so that it can be performed in a single step with reliable results. The protocol covers all steps to obtain the genotypes.