RT Journal Article SR Electronic T1 Two Septin Complexes Mediate Actin Dynamics During Cell Wound Repair JF bioRxiv FD Cold Spring Harbor Laboratory SP 2023.11.14.567084 DO 10.1101/2023.11.14.567084 A1 Stjepić, Viktor A1 Nakamura, Mitsutoshi A1 Hui, Justin A1 Parkhurst, Susan M. YR 2023 UL http://biorxiv.org/content/early/2023/11/16/2023.11.14.567084.abstract AB Cells have robust wound repair systems to prevent further damage or infection and to quickly restore cell cortex integrity when exposed to mechanical and chemical stress. Actomyosin ring formation and contraction at the wound edge are major events during closure of the plasma membrane and underlying cytoskeleton during cell wound repair. Here, we show that all five Drosophila Septins are required for efficient cell wound repair. Based on their different recruitment patterns and knockdown/mutant phenotypes, two distinct Septin complexes, Sep1-Sep2-Pnut and Sep4-Sep5-Pnut, are assembled to regulate actin ring assembly, contraction, and remodeling during the repair process. Intriguingly, we find that these two Septin complexes have different F-actin bending activities. In addition, we find that Anillin regulates the recruitment of only one of two Septin complexes upon wounding. Our results demonstrate that two functionally distinct Septin complexes work side-by-side to discretely regulate actomyosin ring dynamics during cell wound repair.Competing Interest StatementThe authors have declared no competing interest.