RT Journal Article
SR Electronic
T1 Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.11.17.567466
DO 10.1101/2023.11.17.567466
A1 Sanderlin, Allen G.
A1 Margolis, Hannah K.
A1 Meyer, Abigail F.
A1 Lamason, Rebecca L.
YR 2023
UL http://biorxiv.org/content/early/2023/11/17/2023.11.17.567466.abstract
AB Pathogenic bacteria secrete protein effectors to hijack host machinery and remodel their infectious niche. Rickettsia spp. are obligate intracellular bacteria that can cause life- threatening disease, but their absolute dependence on the host cell environment has impeded discovery of rickettsial effectors and their host targets. We implemented bioorthogonal non-canonical amino acid tagging (BONCAT) during R. parkeri infection to selectively label, isolate, and identify secreted effectors. As the first use of BONCAT in an obligate intracellular bacterium, our screen more than doubles the number of experimentally validated effectors for R. parkeri. The novel secreted rickettsial factors (Srfs) we identified include Rickettsia-specific proteins of unknown function that localize to the host cytoplasm, mitochondria, and ER. We further show that one such effector, SrfD, interacts with the host Sec61 translocon. Altogether, our work uncovers a diverse set of previously uncharacterized rickettsial effectors and lays the foundation for a deeper exploration of the host-pathogen interface.Competing Interest StatementThe authors have declared no competing interest.