RT Journal Article SR Electronic T1 The developmental cycle of Dictyostelium discoideum ensures curing of a mycobacterial infection at both cell-autonomous level and by collaborative exclusion JF bioRxiv FD Cold Spring Harbor Laboratory SP 586263 DO 10.1101/586263 A1 Ana Teresa López-Jiménez A1 Monica Hagedorn A1 Matthieu J. Delincé A1 John McKinney A1 Thierry Soldati YR 2019 UL http://biorxiv.org/content/early/2019/03/24/586263.abstract AB During its life cycle, the social amoeba Dictyostelium discoideum alternates between a predatory amoeba and a facultative multicellular form. The single-celled amoeba is a well-established model system to study cell-autonomous mechanisms of phagocytosis and defence against intracellular bacterial pathogens, whereas the multicellular forms are arising as models to study the emergence of innate immune defence strategies. Importantly, during evolution, prokaryotes have also evolved their own strategies to resist predation. Considering these complex ecological relationships, we wondered whether D. discoideum cells infected with intracellular pathogenic mycobacteria would be able to undergo their developmental cycle and what would be the fate of the infection. We show that the combination of cell-autonomous mechanisms and the organisation into a multicellular organism leads to the efficient multistep-curing of a mycobacteria-infected population, thereby ensuring germ-free spores and progeny. Specifically, using a microfluidic device to trap single infected cells, we revealed that in the first curing phase, individual cells rely on three mechanisms to release intracellular bacteria: exocytic release, ejection and lytic release. The second phase occurs at the collective level, when remaining infected cells are excluded from the forming cell aggregates.