RT Journal Article
SR Electronic
T1 Autophagy Restricts Tomato Fruit Ripening Via a General Role in Ethylene Repression
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.12.20.572633
DO 10.1101/2023.12.20.572633
A1 Kumaran, Girishkumar
A1 Pathak, Pradeep Kumar
A1 Quandoh, Ebenezer
A1 Mursalimov, Sergey
A1 Devi, Jyoti
A1 Alkalai-Tuvia, Sharon
A1 Leong, Jia Xuan
A1 Schenstnyi, Kyrylo
A1 Levin, Elena
A1 Üstün, Suayib
A1 Michaeli, Simon
YR 2024
UL http://biorxiv.org/content/early/2024/01/02/2023.12.20.572633.abstract
AB Autophagy, a cellular degradation pathway, and the phytohormone ethylene function in plant development, senescence, and stress responses. However, the manner of their interaction is mostly unknown. We reasoned that this may be revealed by studying autophagy in a climacteric fruit ripening context, for which ethylene is crucial. During ripening, fruits undergo softening, color change, toxic compound degradation, volatile production, and sugar assembly by fine-tuning synthesis and degradation of their cellular content. For autophagy activity assessment, we analyzed autophagy-related 8 (ATG8) lipidation and GFP-ATG8-labeled autophagosome flux in tomato fruit cells. Autophagy activity increased sharply from ripening initiation, climaxed at its middle stage, and declined towards its end, resembling ethylene production dynamics. Silencing the core-autophagy genes SlATG2, SlATG7, and SlATG4 separately in mature fruits resulted in early ethylene production and ripening onset, which was abrogated by 1-methylcyclopropene (1-MCP), an ethylene signaling inhibitor. Beyond ripening, Arabidopsis atg5 and atg7 mutant seedlings exhibited elevated ethylene production and sensitivity to 1-Aminocyclopropane 1-carboxylic acid (ACC), ethylenès precursor, which induces autophagy. This research demonstrates that autophagy limits tomato fruit ripening via a general role in ethylene restriction, opening the path for a mechanistic understanding of autophagy-ethylene crosstalk and harnessing autophagy for fruit shelf-life extension.Competing Interest StatementThe authors have declared no competing interest.