RT Journal Article
SR Electronic
T1 Taste cells expressing Ionotropic Receptor 94e reciprocally impact feeding and egg laying in Drosophila
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.01.23.576843
DO 10.1101/2024.01.23.576843
A1 Guillemin, Jacqueline
A1 Li, Viktoriya
A1 Davis, Grace
A1 Audette, Kayla
A1 Li, Jinfang
A1 Jelen, Meghan
A1 McDowell, Sasha A.T.
A1 Slamani, Samy
A1 Kelliher, Liam
A1 Gordon, Michael D.
A1 Stanley, Molly
YR 2024
UL http://biorxiv.org/content/early/2024/01/23/2024.01.23.576843.abstract
AB Chemosensory cells across the body of Drosophila melanogaster evaluate the environment and play a crucial role in neural circuits that prioritize feeding, mating, or egg laying. Previous mapping of gustatory receptor neurons (GRNs) on the fly labellum identified a set of neurons in L-type sensilla defined by expression of Ionotropic Receptor 94e (IR94e), but the impact of IR94e GRNs on behavior remained unclear. To understand their behavioral output, we used optogenetics and chemogenetics to activate IR94e neurons and found that they drive mild suppression of feeding but enhanced egg laying. In vivo calcium imaging revealed that IR94e GRNs respond strongly to certain amino acids, including glutamate. Furthermore, we found that IR94e is necessary and sufficient for the detection of amino acid ligands, and co-receptors IR25a and IR76b are also required for IR94e GRN activation. Finally, IR94e mutants show behavioral changes to solutions containing amino acids, including increased consumption and decreased egg laying. Overall, our results suggest that IR94e GRNs on the fly labellum discourage feeding and encourage egg laying as part of an important behavioral switch in response to certain chemical cues.Competing Interest StatementThe authors have declared no competing interest.