RT Journal Article SR Electronic T1 BEARscc determines robustness of single-cell clusters using simulated technical replicates JF bioRxiv FD Cold Spring Harbor Laboratory SP 118919 DO 10.1101/118919 A1 DT Severson A1 RP Owen A1 MJ White A1 X Lu A1 B Schuster-Böckler YR 2017 UL http://biorxiv.org/content/early/2017/04/03/118919.abstract AB Technical variance is a major confounding factor in single-cell RNA sequencing, not least because it is not possible to replicate measurements on the same cell. We present BEARscc, a tool that uses RNA spike-in controls to simulate experiment-specific technical replicates. We demonstrate that the tool improves the unsupervised classification of cells and facilitates the biological interpretation of single-cell RNA-seq experiments.